ARF GTPases (TBD) Identification and functional characterization of transcriptional activators in human cells (Nader Alerasool) Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains (Benjamin Piette) A high-density human mitochondrial proximity network (Hana Antonicka) Blencowe lab interactome profiling (Thomas Gonatopoulos-Pournatzis; MS data from Jonathan Roth) Interactome rewiring following pharmacological targeting of BET bromodomains (Jean-Philippe Lambert and Sarah Picaud) Spatial proteomic mapping of human nuclear bodies reveals new functional insights into RNA regulation (Boris J.A. Dyakov) Interactomics uncovers extensive organellar targeting by Zika virus (Etienne Coyaud) Proteomic and structural investigation of phosphorylation-dependent interactions in the core mammalian Hippo pathway (Amber Couzens (proteomics) and Shawn Xiong (structural biology)) Durocher lab interaction proteomics data (Various) A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface (Equal contributions: Étienne Coyaud (proteomics); Gagan D. Gupta, João Gonçalves and Bahareh A. Mojarad (microscopy and cell biology)) The human adenovirus type 5 E4orf4 protein targets two phosphatase regulators of the Hippo signaling pathway (Melissa Z. Mui (Branton lab; manuscript first author) and Yiwang Zhou (Gingras lab; generated all proteomics data)) Mapping the proximity interaction network of the RHO-family GTPases reveals signalling pathways and regulatory mechanisms (Halil Bagci) Proximity based map of proteins involved in mRNA life cycle reveals network of protein complexes in RNA-containing bodies (Jiyoung Youn) Myotubularin-related proteins 3 & 4 interact with PLK1 and CEP55 to control CEP55 recruitment to the midbody (Nicole St-Denis) Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators (Nicole St-Denis (see complete author list under) A quantitative chaperone interaction network for exploring the wiring of cellular protein folding pathways (Mikko Taipale (mikko.taipale@utoronto.ca)) Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition. (Lambert JP*, Ivosev G*, Couzens AL, Larsen B, Taipale M, Lin ZY, Zhong Q, Lindquist S, Vidal M, Aebersold R, Pawson T, Bonner R, Tate S**, Gingras AC**.) Improving the recovery of chromatin-bound protein complexes via chromatin solubilisation in affinity purification coupled to mass spectrometry (Jean-Philippe Lambert) Protein Interaction Network of the Mammalian Hippo Pathway Reveals Mechanisms of Kinase-Phosphatase Interactions (Couzens AL, Knight JDR, Kean MJ, Teo G, Weiss A, Dunham WH, Lin ZY, Bagshaw RD, Sicheri F, Pawson T, Wrana JL, Choi H, Gingras AC)

ARF GTPases (TBD) Identification and functional characterization of transcriptional activators in human cells (Nader Alerasool) Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains (Benjamin Piette) A high-density human mitochondrial proximity network (Hana Antonicka) Blencowe lab interactome profiling (Thomas Gonatopoulos-Pournatzis; MS data from Jonathan Roth) Interactome rewiring following pharmacological targeting of BET bromodomains (Jean-Philippe Lambert and Sarah Picaud) Spatial proteomic mapping of human nuclear bodies reveals new functional insights into RNA regulation (Boris J.A. Dyakov) Interactomics uncovers extensive organellar targeting by Zika virus (Etienne Coyaud) Proteomic and structural investigation of phosphorylation-dependent interactions in the core mammalian Hippo pathway (Amber Couzens (proteomics) and Shawn Xiong (structural biology)) Durocher lab interaction proteomics data (Various) A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface (Equal contributions: Étienne Coyaud (proteomics); Gagan D. Gupta, João Gonçalves and Bahareh A. Mojarad (microscopy and cell biology)) The human adenovirus type 5 E4orf4 protein targets two phosphatase regulators of the Hippo signaling pathway (Melissa Z. Mui (Branton lab; manuscript first author) and Yiwang Zhou (Gingras lab; generated all proteomics data)) Mapping the proximity interaction network of the RHO-family GTPases reveals signalling pathways and regulatory mechanisms (Halil Bagci) Proximity based map of proteins involved in mRNA life cycle reveals network of protein complexes in RNA-containing bodies (Jiyoung Youn) Myotubularin-related proteins 3 & 4 interact with PLK1 and CEP55 to control CEP55 recruitment to the midbody (Nicole St-Denis) Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators (Nicole St-Denis (see complete author list under) A quantitative chaperone interaction network for exploring the wiring of cellular protein folding pathways (Mikko Taipale (mikko.taipale@utoronto.ca)) Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition. (Lambert JP*, Ivosev G*, Couzens AL, Larsen B, Taipale M, Lin ZY, Zhong Q, Lindquist S, Vidal M, Aebersold R, Pawson T, Bonner R, Tate S**, Gingras AC**.) Improving the recovery of chromatin-bound protein complexes via chromatin solubilisation in affinity purification coupled to mass spectrometry (Jean-Philippe Lambert) Protein Interaction Network of the Mammalian Hippo Pathway Reveals Mechanisms of Kinase-Phosphatase Interactions (Couzens AL, Knight JDR, Kean MJ, Teo G, Weiss A, Dunham WH, Lin ZY, Bagshaw RD, Sicheri F, Pawson T, Wrana JL, Choi H, Gingras AC)