Project participants

Amber L Couzens¹, James D. R. Knight¹, Michelle J. Kean^1,2*, Guoci Teo³, Alexander Weiss¹, Wade Dunham¹, Zhen-Yuan Lin¹, Richard D. Bagshaw¹, Frank Sicheri^1,2, Tony Pawson^1,2, Jeffrey L. Wrana^1,2, Hyungwon Choi⁴, and Anne-Claude Gingras^1,2

1 Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
2 Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
3 Department of Statistics and Applied Probability, National University of Singapore.
4 Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore.

* Current address: Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10065.

Address correspondence to Anne-Claude Gingras, gingras@lunenfeld.ca

Contributions

A.L.C. generated 3xFLAG and BirA-FLAG samples, performed MS and validation experiments, designed experiments and analyzed data. J.D.R.K. contributed reagents, discussions and BirA-FLAG control runs. M.J.K. performed initial interaction mapping for MOB1 proteins. G.T. implemented the new SAINT tools and performed data analysis. A.W. provided TEAD-reporter constructs and helped with assay development. W.H.D. contributed BirA-FLAG control runs and assisted with sample preparation. Z.Y.L. helped with mass spectrometric analysis. R.D.B. contributed reagents and discussions. F.S. co-supervised M.J.K., T.P. co-supervised J.D.R.K. and supervised R.D.B., J.W. supervised A.W. and contributed to the functional validation. H.C. and A.-C.G. developed the scoring scheme for MS data. A.-C.G. directed the project. A.L.C. and A-C.G. wrote the manuscript with input from all authors.