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A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface
First Author: Equal contributions: Étienne Coyaud (proteomics); Gagan D. Gupta, João Gonçalves and Bahareh A. Mojarad (microscopy and cell biology)
Abstract:
   The centrosome is the primary microtubule organizing center of the cell and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive interplay between these processes. By monitoring dynamic changes in the centrosome-cilium protein interaction landscape during ciliogenesis, we also identify satellite proteins that support cilia formation. Systematic profiling of proximity interactions combined with functional analysis thus provides a rich resource for better understanding human centrosome and cilia biology. Similar strategies may be applied to other complex biological structures or pathways.

The manuscript has been published in Cell.

The protein interaction data has been highlighted as dataset of the month by the IntAct repository.

This project was co-led by Brian Raught (proteomics) and Laurence Pelletier (centrosome biology and functional screens).


Copyright © 2012 Gingras labs, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital.