DataSets / Project home (A high-density human mitochondrial proximity network)
A high-density human mitochondrial proximity network
First Author: Hana Antonicka
Abstract:
  We used BioID, a proximity-dependent biotinylation assay, to interrogate 100 mitochondrial baits from all mitochondrial sub-compartments to create a high resolution human mitochondrial proximity interaction network. We identified 1465 proteins, producing 15626 unique high confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of mitochondrial compartments, and correlation analysis among preys across all baits allowed us to identify functional clusters involved in diverse mitochondrial functions, and to assign uncharacterized proteins to specific modules. We demonstrate that this analysis can assign isoforms of the same mitochondrial protein to different mitochondrial sub-compartments, and show that some proteins may have multiple cellular locations. Outer membrane baits showed specific proximity interactions with cytosolic proteins and proteins in other organellar membranes, suggesting specialization of proteins responsible for contact site formation between mitochondria and individual organelles. This proximity network will be a valuable resource for exploring the biology of uncharacterized mitochondrial proteins, the interactions of mitochondria with other cellular organelles, and will provide a framework to interpret alterations in sub-mitochondrial environments associated with mitochondrial disease. This dataset is part of a manuscript deposited as a preprint at bioRxiv.

Please contact Anne-Claude Gingras (gingras@lunenfeld.ca) for details on the proteomics dataset and Eric Shoubridge (eric.shoubridge@mcgill.ca) for all other questions.


Navigating this website

• The data included here is by default filtered at a Bayesian false discovery rate of ≤1%, as calculated by SAINTexpress; unselect the "high confidence" buttons to view all data. You can also download a complete interactome table through the Supplementary data section (left).

Select "Explore baits" (left) to view all baits profiled, organized alphabetically (view as a list). Clicking on a bait will retrieve all its interactors (high confidence by default) and indicate which interactions are already deposited in the BioGRID and IntAct databases.

• Selecting multiple baits (or all baits) will enable a download of the SAINT result file that can be opened at ProHits-viz for visualization and further exploration.

• To get a rapid annotation of the gene products on our websites (and on all other websites), you can use GIX, a Gene Information eXtension for Chrome and Firefox.

• Also see the many links to excellent external resources for the baits and/or preys, including ProteinAtlas, ProteomicsDB, NCBI Gene, GeneCards and UniProt.

• Search for a bait or prey by Official Gene Symbol through the "Search" tab on left (or at the top). You will be able to search across all projects you have access to.

Note that the mass spectrometry data has been deposited at MassIVE.


Copyright © 2012 Gingras labs, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital.